Irwin appears in the episode "Prehistoric Ice Man" on the television program the boys are watching. He eventually comes to South Park to help capture Larry for Dr. Alphonse Mephesto and the federal agents. He fails in his attempt, resulting in his death, and Larry escapes in a helicopter to Des Moines.
Irwin also appeared in "Hell on Earth 2006," as a resident of Hell. Satan holds a costume party, mistakes him for a patron at the party wearing a crocodile hunter costume, and deems it offensive. Steve tries to convince Satan that he is the real crocodile hunter, causing Satan to throw him out of his party because he didn't have a costume on. Irwin's appearance in this episode was the source of controversy because it aired only weeks after his actual death by a stingray.
In the episode "Stanley's Cup," Stan tries to convince the Park County Pee-Wee Hockey Team he is coaching that the Denver team won't kill them, and one of the players responds with, "That's what Steve Irwin said about those stingrays," referencing Irwin's cause of death.
His last appearance was in "200" where he, alongside other celebrities, launches a class-action lawsuit against the town of South Park. He still appears with a stingray sticking out of his heart.
He can usually be seen with a stingray in his chest, a direct reference to his accident. He wears tan shorts, a tan coat, and a dark brown hat lined with teeth. He has long blond hair and is rendered with an elongated head.
- "Prehistoric Ice Man" - prominent role where he is seen chasing Larry
- "Hell on Earth 2006" - seen at Satan's party but kicked out.
- "Stanley's Cup" (mentioned only)
- "200" - seen with the other celebrities suing South Park.