Big Gay Al's Animal Sanctuary is a home for homosexual animals of all types and is seen in the Season One episode, "Big Gay Al's Big Gay Boat Ride", where Stan's dog Sparky is revealed to be homosexual.
After Sparky is shown to be homosexual, Stan exclaims in anger that he wishes that he had a masculine dog such as Rin Tin Tin. This offends Sparky, causing him to run away. Sparky stays in this sanctuary before Stan comes back to retrieve him, claiming that he's comfortable with the fact that Sparky is a homosexual. When Stan tries to show everyone else the Sanctuary, it isn't there anymore. All the townsfolk receive their lost animals that had been hiding out here.
Big Gay Al's Animal Sanctuary has yet to reappear on South Park.